| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Validated by Evelyn Kandov (No ORCID)
- Evaluated in human lymph node with CD20 to label B cells. Clone IA6-2 works in several different conjugates to label naive B cells. AF488 and AF647 conjugates are better alternatives for IBEX experiments than AF700.
| Mouse gut: EpCAM (blue, catalog number 118222), IgD (red, catalog number 405730), and CD11c (cyan, catalog number 117312) |
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