UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P02533 Primary Antibody Cytokeratin 14 Human Chicken IgY Poly9060 BioLegend 906004 Unconjugated AB_2616962 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA NA Yes 0000-0002-5187-810X [1] NA 0000-0002-5187-810X  

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

Additional Notes

Human skin: Keratin 14 (yellow, catalog number 906004 and A-32932) and CD44 (purple, catalog number 103016)