| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Evaluated in human lymph nodes and shown to label follicular dendritic cells and B cells. Clone EBVCS-5 has been shown to work in multiple conjugates for IBEX experiments (AF532). Recommend other conjugates over BV421 due to need for photobleaching with light and LiBH4 and high autofluorescence off of 405 nm laser in human tissues.