| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | Novus Biologicals | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [2] | NA | 0000-0003-4379-8967 | 2 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 3 |
Publications
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Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.
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A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.
Additional Notes
- Evaluated in human tonsil FFPE samples with CD20 (clone L26) to label B cell follicles. Does not label follicular dendritic cells in the lymph node. Recommend Abcam ab240987 (clone SP186) in place of this antibody.
- Validated in human follicular lymphoma FFPE samples; amplified with donkey anti-rabbit AF555 (Thermo Fisher Scientific A-31572).
- Validated in human tonsil FFPE samples; amplified with donkey anti-rabbit AF680 (Thermo Fisher Scientific A-10043).