UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q58EE9 Primary Antibody Zrf-1 Zebrafish Mouse IgG1 Monoclonal ZIRC ZDB-ATB-081002-46 Unconjugated AB_10013806 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina GFP transgenic line 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228 1
NA Primary Antibody Zrf-1 Turquoise Killifish Mouse IgG1 Monoclonal ZIRC ZDB-ATB-081002-46 Unconjugated AB_10013806 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina NA 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228  

Publications

  1. A. Kothurkar et al., “Iterative Bleaching Extends Multiplexity (IBEX) imaging facilitates simultaneous identification of all cell types in the vertebrate retina”, bioRxiv, 2024, doi: 10.1101/2024.02.28.582563.

Additional Notes

  1. Prior to IBEX imaging, antigen retrieval was performed by heating the slides for 20 minutes in 10 mM sodium citrate (pH 6). This step quenched the signal in most of the zebrafish GFP transgenic lines tested, but the GFP can be boosted in later rounds of immunolabelling using a primary antibody against GFP. Available through the Zebrafish International Resource Center (ZIRC) but catalog details included via the Zebrafish Information Network (ZFIN).