UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
P11836	Primary Antibody	CD20	Human	Mouse	IgG2a	L26	Thermo Fisher Scientific	42-0202-82	eF615	AB_10853517	Stock	IBEX2D Manual	FFPE	Lymph Node	Metastatic Ovarian Cancer	0.3% Triton-X-100	pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000)	1 mg/ml LiBH4 for more than 120 minutes	Yes	0000-0001-9561-4256	NA	0000-0001-9561-4256	1
P11836	Primary Antibody	CD20	Human	Mouse	IgG2a	L26	Thermo Fisher Scientific	42-0202-82	eF615	AB_10853517	Stock	IBEX2D Manual	FFPE	Tonsil	NA	0.3% Triton-X-100	pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000)	1 mg/ml LiBH4 for more than 120 minutes	Yes	0000-0001-9561-4256	NA	0000-0001-9561-4256	1
H9YXP1	Primary Antibody	CD20	Rhesus Macaque	Mouse	IgG2a	L26	Thermo Fisher Scientific	42-0202-82	eF615	AB_10853517	Stock	Multiplexed 2D Imaging	FFPE	Lymph Node	Infected	0.3% Triton-X-100	pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000)	NA	Yes	0000-0001-9561-4256 [1]	NA	0000-0001-9561-4256	2

Publications

1. M. Pino et al., “Fingolimod retains cytolytic T cells and limits T follicular helper cell infection in lymphoid sites of SIV persistence”, PLoS Pathog ., 15(10):e1008081, 2019, doi:10.1371/journal.ppat.1008081.

Additional Notes

1. IBEX2D Manual is performed on tissue biopsies adhered to glass slides. After imaging, coverslips are soaked off and LiBH4 is placed directly onto the tissues. Conjugate requires extended treatment with 1 mg/ml LiBH4 (120 minutes or more) to extinguish signal. Use in the last cycle or as a fiducial for IBEX experiments.
2. Infected with Simian Immunodeficiency Virus (SIV).