UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670PE PE NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670 PE NA Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

Additional Notes

  1. Clone LN-3 labels professional antigen presenting cells in FFPE tissues from diverse organs. Several conjugates are suitable for IBEX experiments.
  2. Labels macrophages, dendritic cells, and B cells. Good signal in lung FFPE tissue if used at a high concentration.