| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10040 | AF546 | AB_2534016 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11010 | AF546 | AB_2534077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- This antibody can be used to evaluate unconjugated rabbit primary antibodies in a wide range of tissues. Be careful when using unconjugated primary antibodies as IBEX protocol does not strip antibodies. This conjugate does not dye inactivate within 15 minutes of 1 mg/ml LiBH4 treatment. Additional dye inactivation conditions need to be tested.
- This antibody can be used to evaluate unconjugated rabbit primary antibodies in a wide range of tissues. Be careful when using unconjugated primary antibodies as IBEX protocol does not strip antibodies. Place antibodies in first cycle and use direct conjugates in later cycles. This conjugate does not dye inactivate within 15 minutes of 1 mg/ml LiBH4 treatment. Additional dye inactivation conditions need to be tested.