UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967  
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  

Publications

  1. A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.

Additional Notes

  1. Validated in human follicular lymphoma FFPE samples; amplified with donkey anti-rabbit AF594 (Thermo Fisher Scientific A-21207).
Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325)
Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325)