| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Labels cytotoxic T cells in FFPE tonsil tissues. Antibody yields a great signal; however, the Spark YG570 conjugate does not dye inactivate within 15 minutes of 1 mg/ml LiBH4 treatment. Additional dye inactivation conditions need to be tested. This antibody could be placed in the last cycle or used as a fiducial.