| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 1 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 2 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 3 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 4 |
Publications
Additional Notes
- Antibody labels myeloid cells in lymph node as expected. Clone M1/70 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:25 dilution.
- Antibody labels myeloid cells in small intestine as expected. Clone M1/70 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:25 dilution.
- Antibody labels myeloid cells in lymph node as expected. Clone M1/70 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:25 dilution. Works well in 3D imaging as well.
- Antibody labels myeloid cells in small intestine as expected. Clone M1/70 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:25 dilution. Works well in 3D tissue volumes as well.