UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 BioLegend 914204 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0002-3882-457X NA 0000-0002-3882-457X 1

Publications

Additional Notes

  1. Evaluated in human thymus with HLA-DR (clone L243) and shown to label thymic epithelial cells. Clones AE1/AE3 label epithelial cells in diverse tissues in both fixed frozen and FFPE samples. Several conjugates are suitable for IBEX imaging. Custom conjugation by BioLegend.