UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 10D6 Thermo Fisher Scientific MA5-11458 Unconjugated AB_10982556 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
Q86VB7 Primary Antibody CD163 Human Mouse IgG2b EPR19518 Abcam ab213612 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

Additional Notes

  1. Evaluated in human lymph node with HLA-DR (clone L243) to label macrophages. Antibody did not yield expected staining pattern. Clone GHI/61 is recommended for fixed frozen tissues.
  2. Evaluated in human tonsil FFPE samples with HLA-DR (clone LN-3). Antibody yields anticipated spatial pattern and labels macrophages.