| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | EPR19518 | Abcam | ab213612 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Evaluated in human lymph node with HLA-DR (clone L243) to label macrophages. Antibody did not yield expected staining pattern. Clone GHI/61 is recommended for fixed frozen tissues.
- Evaluated in human tonsil FFPE samples with HLA-DR (clone LN-3). Antibody yields anticipated spatial pattern and labels macrophages.