UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Secondary Antibody Mouse IgG2b Human Alpaca IgG2b CTK0105/CTK0106 Thermo Fisher Scientific SA5-10339 AF647 AB_2868386 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

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Additional Notes

  1. This antibody specifically detects mouse IgG2b and can be combined with other mouse IgG isotypes (IgG1, IgG2a) and IgM. Works in diverse tissues and is very suitable for IBEX.