| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Novus Biologicals | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | 1 |
Publications
Additional Notes
- Clone 1A4 labels smooth muscle cells and stroma in diverse tissues. Several conjugates are suitable for IBEX. AF750 conjugate is very dim as compared to preferred conjugates (AF488, eF570, eF660) but does dye inactivate within 15 minutes of LiBH4.