| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 1 |
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 2 |
Publications
Additional Notes
- Poor signal in lymph node at 1:100 dilution. Clone 30-F11 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. This antibody does not work well in 3D, but stains well in 2D.
- Antibody labels immune cells in lymph node as expected. Clone 30-F11 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:100 dilution.