UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 BioLegend 123122 AF647 AB_893480 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Pancreatic Ductal Adenocarcinoma Tumor 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0009-0007-0646-7946 NA 0009-0007-0646-7946 1

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Additional Notes

  1. The AF647 conjugate (123122) works very well and labels tumor-associated macrophages.