UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 2
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 2

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID). This clone works best when conjugated to AF488 or AF647 and used at a 1:50 dilution. Other conjugates (AF594) do not yield as robust signal in our hands.
  2. This clone works best when conjugated to AF488 or AF647 and used at a 1:50 dilution. Other conjugates (AF594) do not yield as robust signal in our hands.
Mouse spleen: CD11c (cyan, catalog number 117312) and CD68 (red, catalog number 137017)
Mouse thymus: CD8 (blue, catalog number 100738) and CD11c (magenta, catalog number 117312)
Mouse lung: CD11c (magenta, catalog number 117312), CD206 (blue, catalog number 141717), and CD31 (green, catalog number 102520)
Mouse lymph node: CD11c (red, catalog number 117312) and CD64 (cyan, catalog number 139322)