UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225 1

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Additional Notes

  1. Labels smooth muscle cells and fibroblasts in Mycobacterium avium infected lungs. Yields good signal to noise in highly autofluorescent tissues.