| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | 1 |
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Additional Notes
- Recommend dilution 1:100. This MHCII antibody labels antigen presenting cells in the tumor microenvironment. In the tumor, the AF647 conjugate (107618) and AF700 (107622) are recommended.