UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
NA	Primary Antibody	B220	Mouse	Rat	IgG2a	RA3-6B2	BD Biosciences	557669	AF488	AB_396781	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967; 0000-0003-1118-7432	NA	0000-0003-4379-8967

Publications

Additional Notes

Mouse lymph node: B220 (cyan, catalog number 557669) and CD3 (red, catalog number 100240)