UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967, 0009-0006-9784-2694 NA 0000-0003-4379-8967 1

Publications

Additional Notes

  1. used with the secondary antibody below in line 5
Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906)