UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
P06800	Primary Antibody	CD45	Mouse	Rat	IgG2b	30-F11	Thermo Fisher Scientific	58-0451-82	AF532	AB_11218871	Stock	Ce3D-IBEX	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	1:10 BD PermWash	NA	1 mg/ml LiBH4 15 minutes	Yes	0009-0003-9817-7874	NA	0009-0003-9817-7874	1
P06800	Primary Antibody	CD45	Mouse	Rat	IgG2b	30-F11	Thermo Fisher Scientific	58-0451-82	AF532	AB_11218871	Stock	Multiplexed 2D Imaging	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	1:10 BD PermWash	NA	NA	Yes	0009-0003-9817-7874	NA	0009-0003-9817-7874	1
P06800	Primary Antibody	CD45	Mouse	Rat	IgG2b	30-F11	Thermo Fisher Scientific	58-0451-82	AF532	AB_11218871	Stock	Ce3D-IBEX	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	1:10 BD PermWash	NA	1 mg/ml LiBH4 15 minutes	Yes	0009-0003-9817-7874	NA	0009-0003-9817-7874	2
P06800	Primary Antibody	CD45	Mouse	Rat	IgG2b	30-F11	Thermo Fisher Scientific	58-0451-82	AF532	AB_11218871	Stock	Multiplexed 2D Imaging	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	1:10 BD PermWash	NA	NA	Yes	0009-0003-9817-7874	NA	0009-0003-9817-7874	2

Publications

Additional Notes

1. Antibody labels immune cells in lymph node as expected. Clone 30-F11 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:100 dilution. Works well in 3D imaging as well.
2. Antibody labels immune cells in lung as expected. Clone 30-F11 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Recommend 1:100 dilution. Works well in 3D imaging as well.