UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q86YL7 Primary Antibody Podoplanin Human Mouse IgG1 D2-40 BioLegend 916601 Unconjugated AB_2565182 Stock IBEX2D Manual FFPE Lung Idiopathic Pulmonary Fibrosis 0.3% Triton-X-100 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C NA Yes 0000-0002-8728-1735 NA 0000-0002-8728-1735 1

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Additional Notes

  1. This antibody labels the anticipated cell type (lymphatic vessel). This antibody was detected using a secondary antibody (Abcam, ab150107). We used a secondary only control to test for non-specific binding of the secondary antibody and observed no signal as expected (not shown).
Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Podoplanin (magenta) and DAPI (blue)