| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Mouse IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21203 | AF594 | AB_141633 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | 1 |
Publications
Additional Notes
- Used to detect PNL-2 (Thermo Fisher Scientific, MSM1-894-P0). Primary antibody did not work but this secondary is excellent. No background.
| Canine melanoma skin FFPE: PNL2 (cyan, catalog number MSM1-894-P0 and A21203) and pan-cytokeratin (blue, catalog number 53-9003-80) |
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