UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102516 AF647 AB_2161029 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303112 AF647 AB_493076 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2
P16284 Primary Antibody CD31 Human Mouse IgG1 C31.3+C31.7+C31.10 Novus Biologicals NBP2-47785AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 3

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)
  2. Clone WM59 labels endothelial cells in various tissues and works in several formats (AF488, AF532, AF647, AF700).
  3. Labels endothelial cells in human FFPE tonsil samples based on patterning and spatial distribution in tissues.
Mouse gut: EpCAM (blue, catalog number 118222), CD31 (magenta, catalog number 102516), and MHCII (green, catalog number 107622)