UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307622 AF647 AB_493177 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 2
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 3
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307622 AF647 AB_493177 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 4

Publications

  1. Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.

Additional Notes

  1. Clone L243 labels professional antigen presenting cells in fixed frozen tissues prepared from diverse organs. Several conjugates are suitable for IBEX experiments.
  2. Clone LN-3 labels professional antigen presenting cells in FFPE tissues from diverse organs. Several conjugates are suitable for IBEX experiments.
  3. Antibody labels the membranes of B cells and myeloid cells in the tonsil. Use at a dilution of 1:100. LN-3 clone is an excellent clone for FFPE tissues and works in many different conjugates.
Human tonsil FFPE: HLA-DR (magenta, catalog number NBP2-47670AF647)
  1. Human jejunum: HLA-DR (pink, catalog number 307622) and EpCAM (blue, catalog number 324228)