UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
NA	Primary Antibody	MHC-II	Mouse	Rat	IgG2b	M5/114.15.2	BioLegend	107622	AF700	AB_493727	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Spleen	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Primary Antibody	MHC-II	Mouse	Rat	IgG2b	M5/114.15.2	BioLegend	107622	AF700	AB_493727	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Thymus	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967
NA	Primary Antibody	MHC-II	Mouse	Rat	IgG2b	M5/114.15.2	BioLegend	107622	AF700	AB_493727	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Primary Antibody	MHC-II	Mouse	Rat	IgG2b	M5/114.15.2	BioLegend	107622	AF700	AB_493727	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Small Intestine	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1

Publications

1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. Validated by Evelyn Kandov (No ORCID)

Mouse spleen: MHCII (blue, catalog number 107622) and F4/80 (magenta, catalog number 123132)

Mouse thymus: CD68 (cyan, catalog number 137017) and MHCII (red, catalog number 107622)

Mouse lung: EpCAM (blue, catalog number 118212), MHCII (magenta, catalog number 107622), and CD169 (green, catalog number 142404)