| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Labels stromal cells in tonsil. Great signal with experimental dye and custom conjugate from BioLegend.
- Labels stromal cells in tonsil. Great signal with experimental dye and custom conjugate from BioLegend. Works beautifully on Cell DIVE imager with IBEX dye inactivation protocol.