UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Novus Biologicals | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Clone L243 labels myeloid cells in many tissues in different conjugates (AF488, AF532, iF594, AF647). AF750 conjugate does not yield a good signal.
- High background. Does not label macrophages, dendritic cells, and B cells in FFPE tissues.