UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 Novus Biologicals NB100-77855AF750 AF750 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670AF750 AF750 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

Additional Notes

  1. Clone L243 labels myeloid cells in many tissues in different conjugates (AF488, AF532, iF594, AF647). AF750 conjugate does not yield a good signal.
  2. High background. Does not label macrophages, dendritic cells, and B cells in FFPE tissues.