UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9BZS2 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 17-4777-42 APC AB_10804651 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0002-2950-2683 NA 0000-0002-2950-2683 1

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Additional Notes

  1. Clone 236A/E7 works well in most fixed frozen tissues but signal is dim in thymus. Amplification with an anti-APC AF647 (R&D Systems FAB8927R) secondary antibody is not sufficient.