| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Primary Antibody | ZO-1 | Zebrafish | Mouse | IgG1 | ZO1-1A12 | Thermo Fisher Scientific | 33-9100 | Unconjugated | AB_2533147 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 [1] | NA | 0009-0000-2047-4228 | 1 |
Publications
- A. Kothurkar et al., “Iterative Bleaching Extends Multiplexity (IBEX) imaging facilitates simultaneous identification of all cell types in the vertebrate retina”, bioRxiv, 2024, doi: 10.1101/2024.02.28.582563.
Additional Notes
- Prior to IBEX imaging, antigen retrieval was performed by heating the slides for 20 minutes in 10 mM sodium citrate (pH 6). This step quenched the signal in most of the zebrafish GFP transgenic lines tested, but the GFP can be boosted in later rounds of immunolabelling using a primary antibody against GFP.