| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
- Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.
Additional Notes
- Validated in human tonsil FFPE samples; labels Tfh cells (colocalizes with PD-1 and CD3); amplified with donkey anti-mouse IgM AF488 (Jackson ImmunoResearch 715-545-020).
- This antibody labels activated T cells and NK cells. Works very well on the Cell DIVE imager with IBEX dye inactivation protocol.