| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | 1 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | 1 |
| G7NCB9 | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | Agilent | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 [1] | NA | 0000-0001-9561-4256 | 2 |
Publications
- M. Pino et al., “Fingolimod retains cytolytic T cells and limits T follicular helper cell infection in lymphoid sites of SIV persistence”, PLoS Pathog ., 15(10):e1008081, 2019, doi:10.1371/journal.ppat.1008081.
Additional Notes
- IBEX2D Manual is performed on tissue biopsies adhered to glass slides. After imaging, coverslips are soaked off and LiBH4 is placed directly onto the tissues. Three exchanges of LiBH4 (15 minute per incubation) are performed to ensure complete removal of signal. Visualized using a donkey anti-rabbit IgG AF647 secondary antibody (Thermo Fisher Scientific A-31573).
- Infected with Simian Immunodeficiency Virus (SIV).