UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P32302 Primary Antibody CD185 (CXCR5) Human Rat IgG2b RF8B2 BD Biosciences 552032 Unconjugated AB_394324 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1

Publications

  1. I. Sayin et al., “Spatial distribution and function of T follicular regulatory cells in human lymph nodes”, J Exp Med., 215(6):1531-1542, 2018, doi:10.1084/jem.20171940.

Additional Notes

  1. Evaluated in human lymph node with CD20 (clone L26). Antibody labels B cell follicles as anticipated (see publication).