| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1130-1899 | NA | 0000-0003-1130-1899 | 1, 2, 3 |
Publications
Additional Notes
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Experimented with reducing fixation time (4h) before putting into sucrose and sectioning it with a cryostat. Longer fixation yields better results.
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This conjugate is a better alternative than AF700.
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The staining was done for 1h at RT using a concentration of 1:50.
| Mouse Spleen: CD3e (white, catalog number 100281) |
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