UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9PTP1 Primary Antibody PCNA Zebrafish Rabbit IgG Polyclonal Proteintech 24036-1-AP Unconjugated AB_2879414 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina GFP transgenic line 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228 1
Q9PTP1 Primary Antibody PCNA Zebrafish Mouse IgG2a PC10 Santa Cruz Biotechnology sc-56 Unconjugated AB_628110 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina GFP transgenic line 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228 1
NA Primary Antibody PCNA Turquoise Killifish Rabbit IgG Polyclonal Proteintech 24036-1-AP Unconjugated AB_2879414 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina NA 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228  
P18248 Primary Antibody PCNA African Clawed Frog (Xenopus laevis) Rabbit IgG Polyclonal Proteintech 24036-1-AP Unconjugated AB_2879414 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina NA 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228  
NA Primary Antibody PCNA Turquoise Killifish Mouse IgG2a PC10 Santa Cruz Biotechnology sc-56 Unconjugated AB_628110 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina NA 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228  
P18248 Primary Antibody PCNA African Clawed Frog (Xenopus laevis) Mouse IgG2a PC10 Santa Cruz Biotechnology sc-56 Unconjugated AB_628110 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina NA 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228  

Publications

  1. A. Kothurkar et al., “Iterative Bleaching Extends Multiplexity (IBEX) imaging facilitates simultaneous identification of all cell types in the vertebrate retina”, bioRxiv, 2024, doi: 10.1101/2024.02.28.582563.

Additional Notes

  1. Prior to IBEX imaging, antigen retrieval was performed by heating the slides for 20 minutes in 10 mM sodium citrate (pH 6). This step quenched the signal in most of the zebrafish GFP transgenic lines tested, but the GFP can be boosted in later rounds of immunolabelling using a primary antibody against GFP.