| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P27597 | Primary Antibody | CD3 | Canine | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | 1 |
Publications
Additional Notes
- Example: Labels T cells in the T cell zone of the lymph node. Used at a dilution of 1:10 to see signal. High background with donkey anti-goat AF555 secondary (A21432). Consider amplifying with opal dyes or TSA-based system or background subtraction with channel arithmetics.
| Canine lymph node FFPE: CD3 (orange, catalog number ab16669 and A21432) |
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