UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q3C2E2 Primary Antibody CD20 Canine Mouse IgG2a L26 Thermo Fisher Scientific 42-0202-82 eF615 AB_10853517 Stock Multiplexed 2D Imaging FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0001-5088-7808 NA 0000-0001-5088-7808 1

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Additional Notes

  1. No signal. Does not label B cells in the lymph node. Samples may have been overfixed (48 hours to 1 week+ in 10% formalin). Intense antigen retrieval (pH 6 and pH 9) was needed for well established antibodies (pan-cytokeratin, clone AE1/AE3).
Canine lymph node FFPE: CD20 (red, catalog number 42-0202-82)