| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Primary Antibody | Alpha smooth muscle actin | Rhesus Macaque | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | 1 |
Publications
Additional Notes
- Labels stroma, fibroblasts and smooth muscle cells, of the spleen. High autofluorescence in AF488 channel. Good signal with 1:50 dilution.
| NHP spleen: CD20 (magenta, catalog number 50-0202-82) and a-SMA (green, catalog number 53-9760-82) |
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