UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
NA	Primary Antibody	Alpha smooth muscle actin	Canine	Mouse	IgG2a	1A4	Thermo Fisher Scientific	41-9760-82	eF570	AB_2573631	Stock	Multiplexed 2D Imaging	FFPE	Skin	Melanoma	0.3% Triton-X-100	pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond	NA	Yes	0000-0001-5088-7808	NA	0000-0001-5088-7808

Publications

Additional Notes

Canine melanoma skin FFPE: a-SMA (magenta, catalog number 41-9760-82) and pan-cytokeratin (green, catalog number 53-9003-80)