UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody CD45.2 Mouse Mouse IgG2a 104 BioLegend 109850 AF594 AB_2629589 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Pancreatic Ductal Adenocarcinoma Tumor 0.3% Triton-X-100 NA NA Yes 0009-0007-0646-7946 NA 0009-0007-0646-7946 1

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Additional Notes

  1. This CD45.2 antibody labels immune cells in Jax#664 strain of C57BL/6 mice expressing the CD45.2 allele. It does not label immune cells in the Taconic NIAID strain#8478 of C57BL/6 mice expressing the CD45.1 allele. This antibody works well in both the AF647 and AF594 conjugates.