UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
NA	Nuclear Dye	JOJO-1	Mouse	NA	NA	NA	Thermo Fisher Scientific	J11372	NA	NA	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Spleen	NA	0.3% Triton-X-100	NA	NA	No	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Nuclear Dye	JOJO-1	Mouse	NA	NA	NA	Thermo Fisher Scientific	J11372	NA	NA	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Thymus	NA	0.3% Triton-X-100	NA	NA	No	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Nuclear Dye	JOJO-1	Mouse	NA	NA	NA	Thermo Fisher Scientific	J11372	NA	NA	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	0.3% Triton-X-100	NA	NA	No	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Nuclear Dye	JOJO-1	Mouse	NA	NA	NA	Thermo Fisher Scientific	J11372	NA	NA	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Small Intestine	NA	0.3% Triton-X-100	NA	NA	No	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
NA	Nuclear Dye	JOJO-1	Mouse	NA	NA	NA	Thermo Fisher Scientific	J11372	NA	NA	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	NA	No	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1

Publications

1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. We used this nuclear dye in the AF532 channel for our IBEX PNAS panels in mouse tissues. This product is discontinued and we now use Hoechst in the 405 channel. We do not recommend this product.