| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P42081 | Primary Antibody | CD86 | Human | Mouse | IgG2b | IT2.2 | BioLegend | 305415 | AF647 | AB_528882 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Evaluated in human lymph node with HLA-DR (clone L243) to label professional antigen presenting cells. Antibody did not yield expected staining pattern. CD86 may be sensitive to detergents in the blocking and staining buffers. This needs to be carefully examined.