UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P13591 Primary Antibody CD56 Human Mouse IgG1 123C3.D5 Novus Biologicals NBP2-33132AF647 AF647 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P13591 Primary Antibody CD56 Human Mouse IgG1 123C3.D5 Novus Biologicals NBP2-33132AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

Additional Notes

  1. Evaluated in human lymph nodes with CD45. Inconclusive results with this clone in multiple formats. Recommend CD94 (clone DX22) for NK cells instead of CD56 in fixed frozen tissues.
  2. Evaluated in human FFPE tonsil samples with CD45. Did not observe a positive signal.