| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103901 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| P08473 | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | R&D Systems | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [2] | NA | 0000-0003-4379-8967 | 3 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 4 |
Publications
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A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.
A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.
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A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.
Additional Notes
- Evaluated in human lymph nodes with follicular lymphoma; labels tumor B cells and colocalizes with CD20 (clone L26). Better signal-to-noise ratio than direct conjugates.
- Evaluated in human follicular lymphoma lymph nodes; labels tumor B cells and colocalizes with CD20 (clone L26). Dim signal. Use CD10 (polyclonal Goat R&D catalog number AF1182) instead.
- Validated in human lymph nodes with follicular lymphoma; labels tumor B cells and colocalizes with CD20 (clone L26). Amplified with donkey anti-goat AF680 (Thermo Fisher Scientific A-21084).
- Amplified with donkey anti-goat DL755 secondary antibody (Thermo Fisher Scientific SA5-10091).