UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P08473 Primary Antibody CD10 Human Mouse IgG1 FR4D11 Caprico Biotechnologies 103901 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P08473 Primary Antibody CD10 Human Rat IgG2b 200103 R&D Systems MAB1126-SP Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 2
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [2] NA 0000-0003-4379-8967 3
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 4

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.

Additional Notes

  1. Evaluated in human lymph nodes with follicular lymphoma; labels tumor B cells and colocalizes with CD20 (clone L26). Better signal-to-noise ratio than direct conjugates.
  2. Evaluated in human follicular lymphoma lymph nodes; labels tumor B cells and colocalizes with CD20 (clone L26). Dim signal. Use CD10 (polyclonal Goat R&D catalog number AF1182) instead.
  3. Validated in human lymph nodes with follicular lymphoma; labels tumor B cells and colocalizes with CD20 (clone L26). Amplified with donkey anti-goat AF680 (Thermo Fisher Scientific A-21084).
  4. Amplified with donkey anti-goat DL755 secondary antibody (Thermo Fisher Scientific SA5-10091).