UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103128 AF700 AB_493715 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11+PD7/26 Novus Biologicals NBP2-34528AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11 + PD7/26 Novus Biologicals NBP2-34528AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Labels immune cells. Beautiful signal in a difficult conjugate. Use at a 1:25 dilution.
  2. Antibody labels membranes of immune cells in FFPE tissues. Strong signal in a difficult conjugate (AF700). Multi-clonal (2B11 + PD7/26) works in many FFPE tissues and conjugates.
Human follicular lymphoma FFPE: CD45 (magenta, catalog number NBP2-34528AF700)