| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- This antibody can be used to evaluate unconjugated goat primary antibodies in a wide range of tissues. Be careful when using unconjugated primary antibodies as IBEX protocol does not strip antibodies. Place antibodies in first cycle and use direct conjugates in later cycles.