UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific SA5-10091 DL755 AB_2556671 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific SA5-10091 DL755 AB_2556671 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

Publications

Additional Notes

  1. This antibody can be used to evaluate unconjugated goat primary antibodies in a wide range of tissues. Be careful when using unconjugated primary antibodies as IBEX protocol does not strip antibodies. Place antibodies in first cycle and use direct conjugates in later cycles.