UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P33681 Primary Antibody CD80 Human Mouse IgG1 2D10 BioLegend 305215 AF647 AB_528874 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

Publications

Additional Notes

  1. Evaluated in human lymph node with HLA-DR (clone L243) to label professional antigen presenting cells. Antibody did not yield expected staining pattern. CD80 may be sensitive to detergents in the blocking and staining buffers. This needs to be carefully examined.