UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody Carbonic anhydrase 1 Zebrafish Rabbit IgG EPR5193 Abcam ab108367 Unconjugated AB_10863424 Stock IBEX2D Manual 4% PFA Fixed Frozen Retina GFP transgenic line 0.1% Triton-X-100 pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker NA Yes 0009-0000-2047-4228 [1] NA 0009-0000-2047-4228 1

Publications

  1. A. Kothurkar et al., “Iterative Bleaching Extends Multiplexity (IBEX) imaging facilitates simultaneous identification of all cell types in the vertebrate retina”, bioRxiv, 2024, doi: 10.1101/2024.02.28.582563.

Additional Notes

  1. Prior to IBEX imaging, antigen retrieval was performed by heating the slides for 20 minutes in 10 mM sodium citrate (pH 6). This step quenched the signal in most of the zebrafish GFP transgenic lines tested, but the GFP can be boosted in later rounds of immunolabelling using a primary antibody against GFP.